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KMID : 1059520090530030279
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Journal of the Korean Chemical Society
2009 Volume.53 No. 3 p.279 ~ p.285
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Preparation of Affinity Column Based on Zr4+ Ion for Phosphoproteins Isolation
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Lee Seon-Mi
Bae In-Ae
Park Jung-Hyen
Kim Tae-Dong
Choi Seong-Ho
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Abstract
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This paper has described about preparation of Zr4+ affinity column based on the poly(styrene-co- glycidyl methacrylate) prepared by emulsion polymerization of styrene and glycidyl methacrylate in order to isolate phosphopeptide. The Zr4+ ions were introduced after the phophonation of an epoxy group on polymeric microspheres. The successful preparation of Zr4+-immobilized polymeric microsphere stationary phase was confirmed through Fourier transform infrared spectra, optical microscopy, scanning electron microscopy, X-ray photoelectron spectra and inductively coupled plasma-atomic emission spectrometer. The separation efficiency for Zr4+ affinity column prepared by slurry packing was tested to phosphonated casein and dephosphonated casein. The resolution time (min) of the phosphonated casein was higher than that of dephosphated casein for Zr4+ affinity polymeric microsphere by liquid chromatography. This Zr4+ affinity column can be used for isolation of phosphonated casein from casein using liquid chromatography.
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KEYWORD
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Zr4+ affinity column, poly(styrene-co-glycidyl methacrylate), polymer microsphere, phosphonated casein, resolution time
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